megatomi.com for Dummies

megatomi.com for Dummies

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Immediately obtain sections as skinny or thick as your software necessitates, without the need of worrying about dropping or distorting worthwhile tissue samples.

Megatome is really a vibrating microtome intended to portion a broad selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome allows substantial-throughput tissue sectioning with uniform floor profile, and negligible tissue damage and information loss.

Antibodies might consider weeks to diffuse via only a few millimeters of tissue, having a steep labeling gradient from surface area to core.

Megatome is designed for accuracy: the blade vibrates at a higher frequency and larger amplitude range than other microtomes, and features a unique deflection Command system.

SE takes advantage of a rotational electric powered industry to disperse really electromobile molecules (for instance antibodies or surfactant micelles) in the course of a porous sample devoid of detrimental electrically charged structures inside the tissue. This enables two-four working day clearing of intact organs,

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them absolutely clear. This permits mild penetration into the sample and makes sure the acquisition of significant-resolution, in-emphasis picture information.

Megatome is often megatomi.com a novel microtome that permits for top-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with negligible tissue hurt and information reduction.

It’s time for you to upgrade your microtome to Megatome. With exact large-frequency slicing for an unmatched number of sample measurements and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for various applications.

The SmartSPIM gentle sheet microscope offers unparalleled resolution and acquisition velocity all through intact tissue volumes, though its modular layout allows you to up grade and customize particular person components.

Docset creation calls for at minimum two possibilities: the identify in the docset and The situation in the Javadoc data files to include during the docset.

This will likely make a docset named Sample in the current Listing. Docset generation could be tailored with optional arguments:

Defend avoids the variability of hydrogel embedding and the data loss from PFA preservation, protecting specimens for several rounds of processing.

Leverage the Obvious+ tissue clearing process, in conjunction with eFLASH and patented stochastic electrotransport systems, to swiftly crystal clear and label full organs. Key highlights and functions incorporate:

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